Tau Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Tau Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB1294394
• Supplier Catalog Number: orb1294394
• Alternative Catalog Number: BYT-ORB1294394-100,BYT-ORB1294394-25
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Conjugation: Unconjugated

Product Description

Rabbit polyclonal antibody to Tau

Product Properties

• Clonality: Polyclonal
• Target: Tau

Application Notes

• Application Dilute: Western Blot 1:3000 - 1:5000 Immunofluorescence 1:200 - 1:500 Immunohistochemistry (Paraffin) 1:200 - 1:300 Immunohistochemistry (Frozen) 1:200 - 1:400

Images

All lanes: Anti-Tau antibody Lysates/proteins at 45 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294394 overnight at 4C.

Anti-Tau antibody Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294394 overnight at 4C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294394 anti-Tau antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescent analysis. Sample: primary cortical neurons, Tau antibody (orb1294394): 1-300, Anti-rabbit 488: 1-500, Fixed: 4% paraformaldehyde at RT for 20 min.

Immunofluorescent analysis. Sample: primary cortical neurons, Tau antibody (orb1294394): 1-300, Anti-rabbit 488: 1-500, Fixed: 4% paraformaldehyde at RT for 20 min.

Immunohistochemical analysis of paraffin embedded Human astroglioma tissue labeling Tau with orb1294394 at 1/300.

Immunohistochemical analysis of paraffin embedded Human brain tissue labeling Tau with orb1294394 at 1/200.

Immunohistochemical analysis of paraffin embedded mouse Alzheimers tissue labeling Tau with orb1294394 at 1/1000 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: Tau antibody (orb1294394): 1-300, Anti-rabbit 488: 1-500, DAPI (blue) was used as the nuclear counter stain.