AIF/Iba1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: AIF/Iba1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB1294405
• Supplier Catalog Number: orb1294405
• Alternative Catalog Number: BYT-ORB1294405-100,BYT-ORB1294405-25
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Conjugation: Unconjugated

Product Description

Rabbit polyclonal antibody to Iba1

Product Properties

• Clonality: Polyclonal
• Target: AIF / Iba1

Application Notes

• Application Dilute: Western Blot 1:500-1:1000 Immunofluorescence 1:100-1:300 Immunohistochemistry (Paraffin) 1:100-1:300 Immunohistochemistry (Frozen) 1:100-1:300

Images

Frozen sectioned for immunohistochemical analysis. Sample: Frozen sectioned Rat brain. Green: Iba1 antibody (orb1294405), Red: TUJ1 antibody, Blue: DAPI.

Iba1/AIF1 antibody - at 1/500 dilution, Lysates at 45 µg per lane, This blot was produced using a 15% SDS-PAGE. PVDF membrane was then blocked with 3% BSA for an hour before being incubated with orb1294405 overnight at 4C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded Rat brain tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: Iba1 antibody (orb1294405): 1-200, Anti-rabbit 488: 1-500. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical of frozen sections. Sample: mouse hippocampus. Green: Iba1 antibody (orb1294405): 1-200, Anti-rabbit 488: 1-500. DAPI (blue) was used as the nuclear counter stain.