RUVBL1/2 ATPase-IN-1 is a potent dual inhibitor of the RUVBL1 and RUVBL2 ATPases, exhibiting IC50 values of 6.0 µM and 7.7 µM, respectively. This compound is a valuable chemical probe for investigating the role of these AAA+ ATPases in oncogenic pathways, supporting cancer research in both cellular and biochemical studies.