5HT4 Receptor Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: 5HT4 Receptor Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB13228
• Supplier Catalog Number: orb13228
• Alternative Catalog Number: BYT-ORB13228-100,BYT-ORB13228-200,BYT-ORB13228-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human 5-HTR4 (21-120/388aa)
• Conjugation: Unconjugated
• Alternative Names: 5-HTR4(5-hydroxytryptamine(serotonin) receptor 4a), Htr4a, 5-HT4, 5-HT4A, 5HT4, Htr45 HT 4, 5 HT4, 5 HT4R, 5 hydroxytryptamine 4 receptor, 5 hydroxytryptamine receptor 4, 5 hydroxytryptamine serotonin receptor 4, 5 hydroxytryptamine4 receptor, 5HT4, 5HT4R, Cardiac 5 HT4 receptor, HTR 4, HTR4, Serotonin 5 HT4 receptor, Serotonin receptor 4, 5HT4R_HUMAN.

Product Description

5HT4 Receptor Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 43 kDa
• UniProt: Q13639
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: HTR4

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-800

Images

IHC-P image of mouse brain tissue using 5HT4 Receptor antibody

IHC-P image of rat brain tissue using 5HT4 Receptor antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (orb13228) at 1:400 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (orb13228) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (orb13228) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Bone (Mouse) Lysate at 40 ug, Primary: Anti-5HT4 Receptor at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 43 kD.

Sample: Lane 1: Stomach (Mouse) Tissue Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Tissue Lysate at 40 ug, Lane 3: Heart (Mouse) Tissue Lysate at 40 ug, Lane 4: Adrenal gland (Mouse) Tissue Lysate at 40 ug, Lane 5: Adrenal gland (Rat) Tissue Lysate at 40 ug, Primary: Anti-5HT4 Receptor (orb13228) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 48 kD.

Sample: Large intestine (Mouse) Lysate at 40 ug, Primary: Anti-5HT4 Receptor (orb13228) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 43 kD.