CD44 Antibody, IgG1, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: CD44 Antibody, IgG1, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB1333592
• Supplier Catalog Number: orb1333592
• Alternative Catalog Number: BYT-ORB1333592-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: FC, IHC, WB
• Species Reactivity: Human
• Immunogen: Full length human recombinant protein of human CD44(NP_000601) produced in HEK293T cell.
• Conjugation: Unconjugated
• Alternative Names: CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1

Product Description

CD44 Antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [OTI1E3]
• Molecular Weight: 79.2 kDa
• Isotype: IgG1
• NCBI: 000610
• UniProt: P16070
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Target: CD44

Application Notes

• Application Dilute: WB 1:400~8000, IHC 1:150, IF 1:100, FLOW 1:50
• Application Notes: Application Notes: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process

Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD44 (RC221771, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD44. P

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD44 monoclonal antibody (HepG2: human, HeLa: human, SVT2: mouse, A549: human, COS7: monkey, Jurkat: human, MDCK: canine, PC12: rat, MCF7: human).

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CD44 mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD44 mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD44 mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CD44 mouse monoclonal antibody.

Anti-CD44 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD44 (RC221771).

Flow cytometric Analysis of living Colo205 cells, using anti-CD44 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).