GLUT12 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: GLUT12 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB13432
• Supplier Catalog Number: orb13432
• Alternative Catalog Number: BYT-ORB13432-100,BYT-ORB13432-200,BYT-ORB13432-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human GLUT12 (251-350/617aa)
• Conjugation: Unconjugated
• Alternative Names: GLUT-12, GTR12_HUMAN, Glucose transporter type 12, Glucose transporter type 12, GLUT 12, GLUT8, SLC2A12, Solute carrier family 2(facilitated glucose transporter) member 12, Solute carrier family 2 facilitated glucose transporter member 12, Solute carrier family 2 member 12.

Product Description

GLUT12 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 68 kDa
• UniProt: Q8TD20
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: SLC2A12

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1µg/Test

Images

IHC-P of rat subcutaneous tumor transplantation using GLUT12 antibody.

A-549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (GLUT12) polyclonal Antibody, Unconjugated (orb13432) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (orb13432) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (orb13432) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (orb13432) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Heart (Rat) Lysate at 40 ug, Primary: Anti-GLUT12 (orb13432) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68kD, Observed band size: 68kD.

SHSY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (GLUT12) polyclonal Antibody, Unconjugated (orb13432) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: mouse heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Glut12 Polyclonal Antibody, Unconjugated (orb13432) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.