MSH3 Human Over-expression Lysate

Product Overview

• Article Name: MSH3 Human Over-expression Lysate
• Biozol Catalog Number: BYT-ORB1345007
• Supplier Catalog Number: orb1345007
• Alternative Catalog Number: BYT-ORB1345007-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Alternative Names: DUP, FAP4, MRP1

Product Description

Transient overexpression lysate of mutS homolog 3 (E. coli) (MSH3)

Product Properties

• Molecular Weight: 127.2 kDa
• NCBI: 002439
• UniProt: P20585
• Form: Components: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer. 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer. 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol
• Target: MSH3

Application Notes

• Application Notes: Application Notes: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48 hours before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20C before shipping