Mitofilin Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Mitofilin Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB13508
• Supplier Catalog Number: orb13508
• Alternative Catalog Number: BYT-ORB13508-100,BYT-ORB13508-200,BYT-ORB13508-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Mitofilin (241-340/758aa)
• Conjugation: Unconjugated
• Alternative Names: Cell proliferation inducing protein 52, Cell proliferation-inducing gene 4/52 protein, HMP, IMMT, IMMT_HUMAN, Inner membrane protein mitochondrial, Inner mitochrondial membrane, MINOS2, Mitochondrial inner membrane organizing system 2, Mitochondrial inner membrane protein, Mitofilin, Motor protein, p87, p87/89, p89, pig4, PIG52, Proliferation-inducing gene 4, MICOS complex subunit MIC60.

Product Description

Mitofilin Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 84 kDa
• UniProt: Q16891
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: IMMT

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500

Images

Immunofluorescence analysis of Human HepG2 cells using IMMT antibody.

Immunofluorescence analysis of Human HepG2 cells using IMMT antibody.

Human HepG2 cells fixed with 3.7% Formaldehyde in medium, blocking performed with 10% NGS in 1x PBS buffer + 0.1% Tween20. Incubated with Anti-Mitofilin/IMMT Polyclonal Antibody (orb13508) at a 1:10 dilution for 1hr at RT, followed by Anti-Rabbit secondary, Cy3 conjugate at a 1:300 dilution incubated at RT for 1hr.

Human HepG2 cells fixed with 3.7% Formaldehyde in medium, blocking performed with 10% NGS in 1x PBS buffer + 0.1% Tween20. Incubated with Anti-Mitofilin/IMMT Polyclonal Antibody (orb13508) at a 1:10 dilution for 1hr at RT, followed by Anti-Rabbit secondary, Cy3 conjugate at a 1:300 dilution incubated at RT for 1hr.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Mitofilin) Polyclonal Antibody, Unconjugated (orb13508) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (itofilin) Polyclonal Antibody, Unconjugated (orb13508) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Mitofilin) Polyclonal Antibody, Unconjugated (orb13508) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A673 (Human) Cell Lysate at 30 ug, Primary: Anti-Mitofilin (orb13508) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68 kD.