KLK1 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: KLK1 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB13525
• Supplier Catalog Number: orb13525
• Alternative Catalog Number: BYT-ORB13525-100,BYT-ORB13525-200,BYT-ORB13525-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human KLK1 (101-200/262aa)
• Conjugation: Unconjugated
• Alternative Names: Kallikrein 1, Kallikrein-1, hK1, Klk6, KLKR, Glandular kallikrein 1, hK 1, Kallikrein serine protease 1, Kidney/pancreas/salivary gland kallikrein, Klk 6, Tissue kallikrein, Klk1b3, Klk1c1, Ngfg, rGK-1, preprokallikrein, KLK1_HUMAN.

Product Description

KLK1 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 26 kDa
• Pubmed: 28615200
• UniProt: P06870
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: KLK1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Western blot of mouse kidney lysate using KLK1 antibody

IHC of rat brain using KLK1 antibody

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (orb13525) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (orb13525) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (orb13525) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (orb13525) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (orb13525) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (orb13525) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human K562 cell lysates, Lane 2: Human SW480 cell lysates, Primary: Anti-KLK1 (orb13525) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 30 kD.