GLUR2 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: GLUR2 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB13619
• Supplier Catalog Number: orb13619
• Alternative Catalog Number: BYT-ORB13619-100,BYT-ORB13619-200,BYT-ORB13619-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human GluR2 (151-250/883aa)
• Conjugation: Unconjugated
• Alternative Names: Ionotropic Glutamate receptor 2, Gria2, Glur-2, GluR-B, Glur2, GluR-2, GluR-K2, Glutamate receptor 2, Glutamate receptor ionotropic, AMPA 2, glutamate receptor, ionotropic, N-methyl D-aspartate 1, NMDA receptor subunit, AMPA-selective glutamate receptor 2, AMPA 2, AMPA 2, AMPA selective glutamate receptor 2, AMPA-selective glutamate receptor 2, AMPA2, GluA2, GLUR 2, GluR B, GluR K2, GluR-2, GluR-B, GluR-K2, GLUR2, GLURB, Glutamate receptor 2, Glutamate receptor ionotropic AMPA 2, Glutamate receptor ionotropic, Gria2, GRIA2_HUMAN, HBGR2.

Product Description

GLUR2 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 97 kDa
• UniProt: P42262
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: GRIA2

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg /test

Images

Blank control: RSC96 (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-AF647 (white blue), Dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 ugl in 100 uL 1X PBS containing 0.5% BSA (green).

Blank control: RSC96 (blue), Isotype Control Antibody: Rabbit IgG-AF647 (orange), Primary Antibody Dilution: 5 uL in 100 uL 1X PBS containing 0.5% BSA (green).

Blank control: RSC96 (blue), Isotype Control Antibody: Rabbit IgG-AF647 (orange), Primary Antibody Dilution: 5 uL in 100 uL 1X PBS containing 0.5% BSA (green).

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (orb13619) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (orb13619) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: HepG2 Cell Lysate at 30 ug, Primary: Anti-GluR2 (orb13619) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 97 kD, Observed band size: 117 kD.

Tissue/Cell: mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GLUR2 Polyclonal Antibody, Unconjugated (orb13619) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Western blot:HepG2 Cell,Primary:Anti-GluR2 at 1:300 dilution.