This kit simultaneously detects both cell death due to both apoptosis and necrosis. It can be used to assess the effects of novel therapeutic agents on cell health. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry.
Samples:
Cell culture
Application Notes:
Application Notes: 1. Prepare samples and controls., 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20., 3. Reconstitute FLICA with 50 µL DMSO., 4. Dilute FLICA 1:5 by adding 200 µL PBS., 5. Add diluted FLICA to each sample at 1:30 - 1:60. For example, to stain at 1:30, add 10 µL to 290 µL of cultured cells. To stain at 1:60, add 5 µL to 295 µL of cultured cells., 6. Incubate approximately 1 hour., 7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells., 8. If desired, label with additional stains, such as Hoechst, 7-AAD, or an antibody., 9. If desired, fix or embed cells., 10. Analyze with a fluorescence microscope, fluorescence platereader, or flow cytometer. SR-FLICA excites at 550-580 nm and emits at 590-600 nm
* VAT and and shipping costs not included. Errors and price changes excepted
