200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Target:
MED21
Analysis of Protein Array containing more than 19000 full-length human proteins using MED21 Mouse Monoclonal Antibody (PCRP-MED21-4B5. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Flow cytometric analysis of PFA-fixed HeLa cells. MED21 Mouse Monoclonal Antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Flow cytometric analysis of PFA-fixed U87 cells. MED21 Mouse Monoclonal Antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MED21 Mouse Monoclonal Antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.
Immunofluorescence Analysis of PFA-fixed U87 cells stained using MED21 Mouse Monoclonal Antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.
SDS-PAGE Analysis. Purified MED21 Mouse Monoclonal Antibody (PCRP-MED21-4B5). Confirmation of Purity and Integrity of Antibody
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