SP100 Antibody, IgG2b, Clone: [PCRP-SP100-1B9], Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: SP100 Antibody, IgG2b, Clone: [PCRP-SP100-1B9], Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB1410458
• Supplier Catalog Number: orb1410458
• Alternative Catalog Number: BYT-ORB1410458-100, BYT-ORB1410458-100, BYT-ORB1410458-20
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant full-length human SP100 protein
• Conjugation: Unconjugated
• Alternative Names: Lysp100b, Nuclear antigen Sp100, Nuclear autoantigen Sp 100, Nuclear dot associated Sp100 protein, SP100, SP100 HMG nuclear autoantigen, SP100 nuclear antigen, Speckled 100 kDa

Product Description

Mouse monoclonal antibody to SP100

Product Properties

• Clonality: Monoclonal
• Clone Designation: [PCRP-SP100-1B9]
• Molecular Weight: 100.4kDa
• Isotype: IgG2b
• UniProt: P23497
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Target: SP100

Images

Analysis of Protein Array containing more than 19000 full-length human proteins using SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Flow cytometric analysis of PFA-fixed HeLa cells. SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using PCRP-SP100-1B9 at 2 ug/ml in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2: HRP-polymer, 30 min. DAB, 5 min.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining using PCRP-SP100-1B9 at 2 ug/ml in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2: HRP-polymer, 30 min. DAB, 5 min.

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.

SDS-PAGE Analysis Purified SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9). Confirmation of Purity and Integrity of Antibody.