LCAT Polyclonal Antibody, Unconjugated, Rabbit

Product Overview

• Article Name: LCAT Polyclonal Antibody, Unconjugated, Rabbit
• Biozol Catalog Number: BYT-ORB1417993
• Supplier Catalog Number: orb1417993
• Alternative Catalog Number: BYT-ORB1417993-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Porcine, Rat
• Conjugation: Unconjugated
• Alternative Names: Phosphatidylcholine-sterol acyltransferase, 2.3.1.43, 1-alkyl-2-acetylglycerophosphocholine esterase, 3.1.1.47, Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase, Platelet-activating factor acetylhydrolase, PAF acetylhydrolase, LCAT

Product Description

Rabbit polyclonal antibody to LCAT.

Product Properties

• Clonality: Polyclonal
• UniProt: P04180

Images

Sample: brain (mouse) Lysate at 40 ug. Primary: Anti- LCAT at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 47kD. Observed band size: 48kD.

Sample: Liver (Mouse) Lysate at 40 ug. Primary: Anti- LCAT at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 47 kD. Observed band size: 49kD.

Sample: K562 (human) Cell Lysate at 40 ug. Primary: Anti- LCAT at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 47kD. Observed band size: 47/52 kD.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LCAT) Polyclonal Antibody, Unconjugated at 1: 200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-LCAT Polyclonal Antibody, Unconjugated 1: 200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LCAT) Polyclonal Antibody, Unconjugated at 1: 200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.