HSP90 Antibody (Biotin), IgG2a, Clone: [4F3.E8], Mouse, Monoclonal

Product Overview

• Article Name: HSP90 Antibody (Biotin), IgG2a, Clone: [4F3.E8], Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB147313
• Supplier Catalog Number: orb147313
• Alternative Catalog Number: BYT-ORB147313-200
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Plant, Rat
• Immunogen: Recombinant Human HSP90 purified from E.coli
• Conjugation: Biotin
• Alternative Names: HSP90, HSP90AB1, HSP90-beta, HSPCB, HSPC2, Heat shock protein HSP 90-beta, Heat shock 84 kDa protein, HSP84, HSP90B

Product Description

Mouse monoclonal to Hsp90 antibody (Biotin)

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [4F3.E8]
• Molecular Weight: 90kDa
• Isotype: IgG2a
• NCBI: 001017963
• UniProt: P07900
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target: HSP90

Application Notes

• Application Dilute: WB (1:2000), IHC (1:100), ICC/IF (1:100)
• Application Notes: Application Notes: 0.5 µg/ml of SMC-149 was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

Images

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Western Blot analysis of Rat tissue lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: muscle tissue. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.