AHA1 Antibody (Biotin), Clone: [25F2.D9], Rat, Monoclonal

Product Overview

• Article Name: AHA1 Antibody (Biotin), Clone: [25F2.D9], Rat, Monoclonal
• Biozol Catalog Number: BYT-ORB147670
• Supplier Catalog Number: orb147670
• Alternative Catalog Number: BYT-ORB147670-100
• Manufacturer: Biorbyt
• Host: Rat
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant Full Length Mouse Aha1 Protein
• Conjugation: Biotin
• Alternative Names: AHSA1, AHA1, HSPC322, p38, C14orf3, LOC10598, Activator of Hsp90 ATPase activity 1, Activator of 90 kDa heat shock protein ATPase homolog 1

Product Description

Rat monoclonal to Aha1 (Biotin). Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding. It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors..

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [25F2.D9]
• Molecular Weight: 38kDa
• NCBI: 666148
• UniProt: Q8BK64
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target: AHA1

Application Notes

• Application Dilute: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000)
• Application Notes: Application Notes: 1 µg/ml of SMC-172 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody

Images

Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.

Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000.

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).

Western Blot analysis of Human Cell lysates showing detection of Aha1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.