NR2B Antibody (Biotin), IgG2b, Clone: [S59], Mouse, Monoclonal

Product Overview

• Article Name: NR2B Antibody (Biotin), IgG2b, Clone: [S59], Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB148756
• Supplier Catalog Number: orb148756
• Alternative Catalog Number: BYT-ORB148756-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: AM, ICC, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B
• Conjugation: Biotin
• Alternative Names: NMDAR2B, hNR3, MGC142180, glutamate receptor subunit epsilon-2, NR3

Product Description

Mouse monoclonal antibody against GRIN2B conjugated to Biotin

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [S59]
• Molecular Weight: 166kDa
• Isotype: IgG2b
• NCBI: 036706
• UniProt: Q00960
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target: NR2B

Application Notes

• Application Dilute: WB (1:1000), IHC (1:1000), ICC/IF (1:100)
• Application Notes: Application Notes: 1 µg/ml of SMC-337 was sufficient for detection of NR2B in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody

Images

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain, Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2B/NR2B Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.

Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.

Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.