GIT1 Antibody (APC), IgG1, Clone: [N39B/8 Formerly sold as S39B-8], Mouse, Monoclonal

Product Overview

• Article Name: GIT1 Antibody (APC), IgG1, Clone: [N39B/8 Formerly sold as S39B-8], Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB150047
• Supplier Catalog Number: orb150047
• Alternative Catalog Number: BYT-ORB150047-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: ICC, IF, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
• Conjugation: APC
• Alternative Names: ARF GAP GIT1, ARF GTPase-activating protein, ARF GTPase-activating protein GIT1, CAT1, Cool-associated and tyrosine-phosphorylated protein 1, G protein-coupled receptor kinase-interactor 1, GIT 1, GIT1, GRK-interacting protein 1

Product Description

Mouse monoclonal to GIT1 (APC). G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration. GIT-1 has also been implicated in neuronal functions including synapse formation and the pathology of Huntington disease. Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains. Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology..

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [N39B/8 Formerly sold as S39B-8]
• Molecular Weight: 90kDa
• Isotype: IgG1
• NCBI: 114002
• UniProt: Q9Z272
• Buffer: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Target: GIT1

Application Notes

• Application Dilute: WB (1:1000)
• Application Notes: Application Notes: 1 µg/ml of SMC-413 was sufficient for detection of GIT1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody