HO-1 Antibody (Biotin), Rabbit, Polyclonal

Product Overview

• Article Name: HO-1 Antibody (Biotin), Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB151360
• Supplier Catalog Number: orb151360
• Alternative Catalog Number: BYT-ORB151360-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, IHC, WB
• Species Reactivity: Canine, Human, Mouse, Rat
• Immunogen: Human heme-oxygenase (HO-1) synthetic multiple antigenic peptide
• Conjugation: Biotin
• Alternative Names: HSP32, HMOX1, Heme oxygenase 1, HO, HO1, 32 kD, bK286B10, D8Wsu38e, heat shock protein 32 kD, heat shock protein 32kD, Heat shock protein, Heme oxygenase (decycling) 1, Hemox, HMOX 1, Hmox, Hmox1, HMOX1_HUMAN, HO 1, HO-1, Heme Oxygenase-1, Haem Oxygenase-1, Heat Shock Protein 32, Heme Oxygenase (Decyclizing), EC 1.14.14.18

Product Description

Rabbit polyclonal to HO-1 (Biotin). Heme-oxygenase is a ubiquitous enzyme that catalyzes Heme-oxygenase is a ubiquitous enzyme that catalyzes...

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 32kDa
• NCBI: 002124
• UniProt: P09601
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Target: HO-1

Application Notes

• Application Dilute: WB (1:1000), ICC/IF (1:100)
• Application Notes: Application Notes: 1 µg/ml of SPC-112 was sufficient for detection of HO-1 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody

Images

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HO-1 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 100x. Heat Shocked at 42C for 1h.

Western blot analysis of Human Cell line lysates showing detection of HO-1 protein using Rabbit Anti-HO-1 Polyclonal Antibody. Load: 15 µgprotein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HO-1 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite. Heat Shocked at 42C for 1h.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HO-1 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite.