Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Form:
Whole IgG
Target:
NS1-BP
Application Dilute:
WB - 1:2,000 - 1:10,000, IP - 2 - 5 µg/mg lysate
Application Notes:
Application Notes: Format: Whole IgG
Detection of human and mouse NS1-BP by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB, 1 mg for IP, 20% of IP loaded) , HEK293T (T, 50 µg) , and mouse NIH 3T3 (M, 50 µg) cells. Antibodies: Affinity purified rabbit anti-NS1-BP antibody orb1525831 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NS1-BP was also immunoprecipitated by rabbit anti-NS1-BP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
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