Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Form:
Whole IgG
Target:
UBE2O
Application Dilute:
WB - 1:2,000 - 1:10,000, IP - 2 - 5 µg/mg lysate
Application Notes:
Application Notes: Format: Whole IgG
Detection of human UBE2O by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBE2O antibody orb1527637 used for IP at 3 µg per reaction.
Detection of human UBE2O by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE2O antibody orb1527637 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of mouse UBE2O by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE2O antibody orb1527637 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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