Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Form:
Whole IgG
Target:
BRG1/SMARCA4
Application Dilute:
WB - 1:2,000 - 1:10,000, IP - 2 - 10 µg/mg lysate, IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Application Notes:
Application Notes: Format: Whole IgG
Detection of mouse BRG1/SMARCA4 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 used at a dilution of 1:1, 000 (0.2µg/ml).
Detection of human BRG1/SMARCA4 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-BRG1/SMARCA4 antibody.
Detection of human BRG1/SMARCA4 by western blot.Samples: Whole cell lysate from Jurkat, K-562, HEK293T, MCF-7, and HeLa cells. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody at 0.04 µg/ml.
Detection of mouse BRG1/SMARCA4 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody at 0.04 µg/ml.
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