This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
Conjugation:
Unconjugated
Alternative Names:
MSLN, CAK1 antigen, MPF, SMRP, Mesothelin, CAK1
MSLN/Mesothelin Antibody
Clonality:
Monoclonal
Concentration:
1 mg/ml
Clone Designation:
[MN-1]
Isotype:
IgG2a
Buffer:
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Further information: This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kD in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23 C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.
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