CD63 Antibody (Biotin), Clone: [MEM-259], Monoclonal

Product Overview

• Article Name: CD63 Antibody (Biotin), Clone: [MEM-259], Monoclonal
• Biozol Catalog Number: BYT-ORB154462
• Supplier Catalog Number: orb154462
• Alternative Catalog Number: BYT-ORB154462-0.1
• Manufacturer: Biorbyt
• Category: Antikörper
• Application: FC, ICC, IHC-P, IP
• Species Reactivity: Human
• Immunogen: HPB-ALL T cell line
• Conjugation: Biotin
• Alternative Names: OMA81H, Granulophysin, Tetraspanin-30, Tspan-30, MLA1, ME491, LAMP-3, OMA81H, TSPAN30

Product Description

Mouse monoclonal antibody conjugated to Biotin which recognizes is a CD63 that is lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is observed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. CD63 is belongs to the TM4 superfamily of leukocyte glycoproteins. CD63 may participates in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome.

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [MEM-259]
• UniProt: P08962
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Target: CD63

Application Notes

• Application Notes: Application Notes: Flow cytometry: Recommended dilution: 1-3 µg/ml, positive material: activated platelets, neutrophils and basophils

Images

Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0.6 µg/ml, Streptavidin APC) and anti-human CD203c (NP4D6) PE antibody (20 µl reagent / 100 µl of peripheral whole blood).

Separation of CD63 positive CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of IgE stimulated human peripheral whole blood using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0.6 µg/ml, Streptavidin APC).

Flow cytometry surface staining pattern of IgE stimulated human peripheral whole blood stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0.6 µg/ml, Streptavidin APC).