Factor VIII B chain Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Factor VIII B chain Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB156821
• Supplier Catalog Number: orb156821
• Alternative Catalog Number: BYT-ORB156821-100,BYT-ORB156821-200,BYT-ORB156821-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human
• Immunogen: KLH conjugated synthetic peptide derived from human Factor VIII B chain (1101-1200/2351aa)
• Conjugation: Unconjugated
• Alternative Names: coagulation factor VIII, Ahf, Antihemophilic factor, Coagulation factor VIII, Coagulation factor VIII associated protein b, Coagulation factor VIII isoform b, Coagulation factor VIII procoagulent component, Coagulation factor VIIIc, Dna segment on chromosome x unique 1253 expressed sequence, Dxs1253e, F8, F8 protein, F8b, F8c, Factor VIII F8b, FactorVIII, FVIII, Hema coagulation factor VIIIc procoagulent component, Hemophilia a, Hemophilia classic, OTTHUMP00000061446, Procoagulant component, FVIII-Ag, Factor VIIIa heavy chain, 200 kDa isoform, Factor VIIIa heavy chain, 92 kDa isoform, Factor VIII B chain, AHF, DXS1253E, F8B, F8C, FVIII, HEMA, FA8_HUMAN.

Product Description

Factor VIII B chain Rabbit Polyclonal Antibody. FVIII is a large molecule glycoprotein composed of 2,332 amino acids and is divided into multiple functional domains (arranged in the order of A1-A2-B-A3-C1-C2).

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 138 kDa
• UniProt: P00451
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: F8

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Factor VIII B chain) Polyclonal Antibody, Unconjugated (orb156821) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Factor VIII B chain) Polyclonal Antibody, Unconjugated (orb156821) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Factor VIII B chain) Polyclonal Antibody, Unconjugated (orb156821) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Factor VIII B chain) Polyclonal Antibody, Unconjugated (orb156821) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Factor VIII B chain) Polyclonal Antibody, Unconjugated (orb156821) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-Factor VIII B chain (orb156821) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110 kD, Observed band size: 110 kD.

Sample: Siha Cell (Human) Lysate at 40 ug, Primary: Anti-Factor VIII B chain (orb156821) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 138kD, Observed band size: 135kD.

IHC-P analysis of human brain using Factor VIII B chain Polyclonal Antibody at 1:200 dilution.

Western blot:Siha Cell(Human),Primary:Anti-Factor VIII B chainat 1:300 dilution.