Mst2 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Mst2 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB157906
• Supplier Catalog Number: orb157906
• Alternative Catalog Number: BYT-ORB157906-100,BYT-ORB157906-200,BYT-ORB157906-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Mst2 (351-450/491aa)
• Conjugation: Unconjugated
• Alternative Names: KRS2, Mammalian STE20 like protein kinase 2, MST 2, MST2, Serine/threonine protein kinase 4, Serine/threonine protein kinase Krs 2, STE20 like kinase MST2, STK 4, STK4, STK3_HUMAN, Serine/threonine-protein kinase 3, Mammalian STE20-like protein kinase 2, MST-2, STE20-like kinase MST2, Serine/threonine-protein kinase Krs-1, Serine/threonine-protein kinase 3 36kDa subunit, MST2/N, Serine/threonine-protein kinase 3 20kDa subunit, MST2/C.

Product Description

Mst2 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 54 kDa
• UniProt: Q13043
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: STK3

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500

Images

Immunohistochemical staining of human breast carcinoma tissue using Mst2 antibody.

Immunohistochemical staining of Human prostate tissue using Mst2 antibody.

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Mst2) polyclonal Antibody, Unconjugated (orb157906) 1:25, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Mst2) Polyclonal Antibody, Unconjugated (orb157906) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Mst2) Polyclonal Antibody, Unconjugated (orb157906) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Mst2) Polyclonal Antibody, Unconjugated (orb157906) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: human breast carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Mst2 Polyclonal Antibody, Unconjugated (orb157906) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human breast carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Mst2 Polyclonal Antibody, Unconjugated (orb157906) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Human laryngeal carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Mst2 Polyclonal Antibody, Unconjugated (orb157906) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.