Application Notes: This protein carries no tag. The protein has a calculated MW of 13.5 kDa. The protein migrates as 13-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Immobilized Human BDNF, premium grade (Cat. No. orb1675341) at 1 µg/mL (100 µL/well) can bind Human TrkB, His Tag (Cat. No. orb257719) with a linear range of 2-78 ng/mL (QC tested).
Human BDNF Protein (HEK293), premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% .
iPSC derived human brain organoids, expressing neuron marker TUJ1 (green) and neuron stem cell marker NESTIN (red), were cultured for 36 days with BDNF (orb1675341) and GDNF (orb1675340). H&E staining showed brain organoids had classic neuron stem cell rossettes histology.
iPSC derived neurons forming axon net-works were cultured with differentiation protocol, including BDNF (orb1675341) and GDNF (orb1675340).
Immobilized Human BDNF Protein (HEK293), premium grade (orb1675341) at 1 µg/mL (100 µL/well) can bind Human TrkB, His Tag (orb257719) with a linear range of 2-78 ng/mL (QC tested).
Immobilized Human BDNF Protein (HEK293), premium grade (orb1675341) at 2 µg/mL (100 µL/well) can bind Human TrkB, Fc Tag (orb257720) with a linear range of 1-20 ng/mL (Routinely tested).
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