0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
TMPRSS2
Application Dilute:
WB=1:500-2000, ELISA=1:5000-10000
Measured by its binding ability in a indirect ELISA. Immobilized Human TMPRSS2 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human TMPRSS2 Antibody, the EC50 is 118.5 ng/ml.
Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human HeLa cell lysates, Primary: Anti-TMPRSS2 (orb1675350) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 58 kDa.
Western blot:Lane 1:Human HepG2 cell,Lane 2:Human HeLa cell,Primary:Anti-TMPRSS2 at 1:1000 dilution.
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