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Sample: Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag), Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag), Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag), Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag), Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag), Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag), Primary: Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid (orb1675359) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 46 kDa, Observed band size: 50 kDa. |