Rabbit anti-JMJD6 Recombinant Monoclonal Antibody, Clone: [BLR254L], Unconjugated

Product Overview

• Article Name: Rabbit anti-JMJD6 Recombinant Monoclonal Antibody, Clone: [BLR254L], Unconjugated
• Biozol Catalog Number: BYT-ORB1710831
• Supplier Catalog Number: orb1710831
• Alternative Catalog Number: BYT-ORB1710831-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: Between 125 and 175
• Conjugation: Unconjugated
• Alternative Names: peptide-lysine 5-dioxygenase JMJD6, PTDSR1, PTDSR, PSR, phosphatidylserine receptor, lysyl-hydroxylase JMJD6, jumonji domain-containing protein 6, jumonji domain containing 6, jmjC domain-containing protein 6, histone arginine demethylase JMJD6, bifunctional arginine demethylase and lysyl-hydroxylase JMJD6, arginine demethylase and lysine hydroxylase, Protein PTDSR

Product Description

JMJD6 Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1000 µg/ml
• Clone Designation: [BLR254L]
• UniProt: Q6NYC1
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Form: Whole IgG
• Target: JMJD6

Application Notes

• Application Dilute: WB: 1:1,000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Application Notes: Application Notes: Format: Whole IgG

Images

Detection of human JMJD6 (shaded) in A549 cells by flow cytometry. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of mouse JMJD6 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of human JMJD6 by immunocytochemistry. Sample: FFPE section of human A549 cells. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse JMJD6 by immunocytochemistry. Sample: FFPE section of mouse ND7/23 cells. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human JMJD6 by immunohistochemistry. Sample: FFPE section of human testis. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse JMJD6 by immunohistochemistry. Sample: FFPE section of mouse testis. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse JMJD6 by western blot. Samples: Whole cell lysate (10 µg) from BW5147.3, NIH 3T3, CH27, ND7/23, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human JMJD6 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Hep-G2, RKO, and OVCAR-8 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710831) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.