Rabbit anti-KLF5 Recombinant Monoclonal Antibody, Clone: [BLR243L], Unconjugated

Product Overview

• Article Name: Rabbit anti-KLF5 Recombinant Monoclonal Antibody, Clone: [BLR243L], Unconjugated
• Biozol Catalog Number: BYT-ORB1733253
• Supplier Catalog Number: orb1733253
• Alternative Catalog Number: BYT-ORB1733253-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IHC, IP, WB
• Species Reactivity: Human, Mouse
• Immunogen: Between 325 and 375
• Conjugation: Unconjugated
• Alternative Names: GC-box-binding protein 2, Kruppel-like factor 5 (intestinal), Krueppel-like factor 5, Klf5C isoform, intestinal-enriched kruppel-like factor, intestinal-enriched krueppel-like factor, intestinal Kruppel-like factor, IKLF, epididymis secretory sperm binding protein, colon kruppel-like factor, colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2, basic transcription element binding protein 2, transcription factor BTEB2, GC box binding protein 2

Product Description

KLF5 Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1000 µg/ml
• Clone Designation: [BLR243L]
• UniProt: Q13887
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Form: Whole IgG
• Target: KLF5

Application Notes

• Application Dilute: WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Application Notes: Application Notes: Format: Whole IgG

Images

Detection of mouse KLF5 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of human KLF5 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of human KLF5 by immunocytochemistry. Sample: FFPE section of HT-29 cells. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse KLF5 by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human KLF5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse KLF5 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human KLF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from KM12 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) used for IP at 6 µl/mg lysate.

Detection of mouse KLF5 by western blot. Samples: Whole cell lysate (25 µg) from CT26, WEHI-231, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human KLF5 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, SW620, MCF-7, and KM12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.