5-TCO-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting TCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free Click Chemistry (TCO-Tetrazine-Ligation) that offers the choice: to introduce a Biotin group (via Tetrazines of Biotin) for subse-quent purification tasks, to introduce fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging, to crosslink the DNA to Tetrazine-functionalized biomolecules e.g. proteins.
solution in water, Color: colorless to slightly yellow, pH: 7.5 0.5
Formula:
C32H53N4O21P3
Application Notes:
Application Notes: Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: max 240 nm, 10.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5)
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