Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Target:
CD63
Elisa plates were pre-coated with 0.5 µg/per well purified human CD63 full length membrane nanoparticles. Serial diluted anti-CD63 monoclonal antibody (orb1743123) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CD63 monoclonal antibody binding with CD63 full length membrane nanoparticles is 61.65 ng/ml.
FACS analysis of CD63 MNPs A. Negative Control 1: CD63 full length membrane nanoparticles samples were stained only with Goat anti-human lgG 488 secondary antibody. B. Negative Control 2: Control membrane nanoparticles samples were stained with anti-CD63 antibody (orb1743123) at 2 µg/mL, followed by Goat anti-human IgG 488 secondary antibody. C. Negative Control 3: CD63 full length membrane nanoparticles samples were stained with anti-GPRC5D antibody (an irrelevant antibody) at 2 µg/mL, followed by Goat anti-human IgG 488 secondary antibody. D. CD63 full length membrane nanoparticles samples were stained with anti-CD63 antibody (orb1743123) at 2 µg/mL, followed by Goat anti-human IgG 488 secondary antibody.
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