Human GPR75 full length protein-synthetic nanodisc

Catalog Number: BYT-ORB1743239
Article Name: Human GPR75 full length protein-synthetic nanodisc
Biozol Catalog Number: BYT-ORB1743239
Supplier Catalog Number: orb1743239
Alternative Catalog Number: BYT-ORB1743239-10,BYT-ORB1743239-100,BYT-ORB1743239-50
Manufacturer: Biorbyt
Category: Proteine/Peptide
Species Reactivity: Human
Alternative Names: GPRchr2, WI31133
Human GPR75 full length protein-synthetic nanodisc
Molecular Weight: The human full length GPR75 protein has a MW of 59.4 kDa
UniProt: O95800
Form: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lowe
Target: GPR75
Elisa plates were pre-coated with Flag Tag GPR75-Nanodisc (0.2 µg/per well). Serial diluted anti-GPR75 monoclonal antibody (orb1471689) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-GPR75 monoclonal antibody binding with GPR75-Nanodisc is 303.6 ng/ml.
WB analysis of Human GPR75-Nanodisc with anti-Flag monoclonal antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution