Human TNFRSF1B Protein, hFc Tag

Product Overview

• Article Name: Human TNFRSF1B Protein, hFc Tag
• Biozol Catalog Number: BYT-ORB1743387
• Supplier Catalog Number: orb1743387
• Alternative Catalog Number: BYT-ORB1743387-10,BYT-ORB1743387-100,BYT-ORB1743387-50
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD120b,p75,p75TNFR,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1B,TNFR2,TNFR80

Product Description

Recombinant Human TNFRSF1B Protein with C-terminal human Fc tag

Product Properties

• Molecular Weight: The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide. The apparent molecular mass of TNFRSF1B-hFc is approximately 55-70kDa due to glycosylation.
• UniProt: P20333
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Target: TNFRSF1B

Images

Human B7-2 Protein, hFc Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human B7-2, hFc tagged protein (orb689458) can bind Human CTLA4, mFc-His tagged protein orb689378 in a linear range of 0.048-2.694 ng/ml.

ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human B7-2, hFc tagged protein (orb689458) can bind Human CD28, mFc-His tagged protein orb689375 in a linear range of 62.5-4000 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human CD28 (A) were surface stained with Human B7-2, hFc tagged protein (orb689458) 1 µg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.