IFN gamma Antibody (Biotin), Clone: [4S.B3], Monoclonal

Product Overview

• Article Name: IFN gamma Antibody (Biotin), Clone: [4S.B3], Monoclonal
• Biozol Catalog Number: BYT-ORB179917
• Supplier Catalog Number: orb179917
• Alternative Catalog Number: BYT-ORB179917-0.1
• Manufacturer: Biorbyt
• Category: Antikörper
• Application: ELISA, FC, ICC, IHC-P, IP, WB
• Species Reactivity: Human, Primate
• Immunogen: Interferon gamma derived from human leukocytes
• Conjugation: Biotin
• Alternative Names: Interferon gamma, IFN-gamma

Product Description

Mouse monoclonal antibody against IFN gamma conjugated to Biotin

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [4S.B3]
• UniProt: P01579
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Target: IFN gamma

Application Notes

• Application Notes: Application Notes: Flow cytometry: Recommended dilution 1-4 µg/ml. Intracellular staining.ELISA: Detection antibody in combination with capture antibody NIB42

Images

Cytokine IFN gamma (IFNƴ) sandwich ELISA (enzyme-linked immunosorbent assay) that utilizes antibody clone NIB42 as the protein capture antibody and antibody clone 4S.B3-Biotin as the detection antibody. Recombinant IFNƴ was captured by NIB42 and detected by variable amounts of 4S.B3-Biotin. The 4S.B3-Biotin conjugate was visualized by Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of cytokine and to the amount of the detection antibody.

Flow cytometry multicolor intracellular staining pattern of human lymphocytes (PHA stimulated and Brefeldin A + Monesin treated) stained using anti-human IFN-gamma (4S.B3) biotin antibody (concentration in sample 1.7 µg/ml, Streptavidin APC) and anti-human CD3 (UCHT1) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).

Separation of human IFN-gamma positive CD3 positive lymphocytes (red-filled) from IFN-gamma negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood (PHA stimulated and Brefeldin A + Monesin treated) stained using anti-human IFN-gamma (4S.B3) biotin antibody (concentration in sample 1.7 µg/ml, Streptavidin APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood (PHA stimulated and Brefeldin A + Monesin treated) stained using anti-human IFN-gamma (4S.B3) biotin antibody (concentration in sample 1.7 µg/ml, Streptavidin APC).