Detection of human PVRIG by western blot. Samples: Whole cell lysate (25 µg) from CCRF-CEM, A-549, Jurkat, C5/MJ, and Ramos cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRIG recombinant monoclonal antibody (orb1806426) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.
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