Rabbit anti-ARID1B Recombinant Monoclonal Antibody, Clone: [BLR291L], Unconjugated

Product Overview

• Article Name: Rabbit anti-ARID1B Recombinant Monoclonal Antibody, Clone: [BLR291L], Unconjugated
• Biozol Catalog Number: BYT-ORB1806438
• Supplier Catalog Number: orb1806438
• Alternative Catalog Number: BYT-ORB1806438-10
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IHC, IP, WB
• Species Reactivity: Human, Mouse
• Immunogen: Between 1425 and 1475
• Conjugation: Unconjugated
• Alternative Names: CSS1, MRD12, Osa homolog 2, P250R, hOsa2, ELD/OSA1, ELD (eyelid)/OSA protein, DAN15, OSA2, BRG1-binding protein hELD/OSA1, BRG1-binding protein ELD/OSA1, BRG1-associated factor 250b, BAF250B, AT-rich interactive domain-containing protein 1B, AT rich interactive domain 1B (SWI1-like), ARID domain-containing protein 1B, 6A3-5, BRIGHT

Product Description

Rabbit anti-ARID1B Recombinant Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1000 µg/ml
• Clone Designation: [BLR291L]
• UniProt: Q8NFD5
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Form: Whole IgG
• Target: ARID1B

Application Notes

• Application Dilute: WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Application Notes: Application Notes: Format: Whole IgG

Images

Detection of mouse ARID1B (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1B (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1B by immunocytochemistry. Sample: FFPE section of RKO cells. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse ARID1B by immunocytochemistry. Sample: FFPE section of ND7/23 cells. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human ARID1B by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse ARID1B/BAF250 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438) used for IP at 6 µl/mg lysate.

Detection of mouse ARID1B by western blot. Samples: Whole cell lysate (25 µg) from RenCa, EL4, A20, J774A1, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human ARID1B by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, U2OS, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.