Rabbit anti-ARID1A/BAF250 Recombinant Monoclonal Antibody, Clone: [BLR279L], Unconjugated

Product Overview

• Article Name: Rabbit anti-ARID1A/BAF250 Recombinant Monoclonal Antibody, Clone: [BLR279L], Unconjugated
• Biozol Catalog Number: BYT-ORB1806458
• Supplier Catalog Number: orb1806458
• Alternative Catalog Number: BYT-ORB1806458-10
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IHC, IP, WB
• Species Reactivity: Human, Mouse
• Immunogen: Between 750 and 800
• Conjugation: Unconjugated
• Alternative Names: osa homolog 1, SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1, hOSA1, hELD, MRD14, OSA1, OSA1 nuclear protein, P270, ELD, SWI/SNF complex protein p270, BRG1-associated factor 250, SWI-like protein, SMARCF1, B120, ARID domain-containing protein 1A, C1orf4, AT-rich interactive domain-containing protein 1A, CSS2, BAF250, BAF250a, BM029, brain protein 120, BRG1-associated factor 250a, chromatin remodeling factor p250, AT rich interactive domain 1A (SWI-like)

Product Description

Rabbit anti-ARID1A/BAF250 Recombinant Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 50 µg/ml
• Clone Designation: [BLR279L]
• UniProt: O14497
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Form: Whole IgG
• Target: ARID1A/BAF250

Application Notes

• Application Dilute: WB: 1:1000, IP: 20 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Application Notes: Application Notes: Format: Whole IgG

Images

Detection of human ARID1A/BAF250 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806458) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of mouse ARID1A/BAF250 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti- ARID1A recombinant monoclonal antibody (orb1806458) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1A/BAF250 by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806458). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse ARID1A/BAF250 by immunocytochemistry. Sample: FFPE section of mouse mIMCD-3 cells. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806458). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human ARID1A/BAF250 by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806458). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse ARID1A/BAF250 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806458). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human ARID1A/BAF250 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP, 10% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806458) used for IP at 20 µl/mg lysate.

Detection of mouse ARID1A/BAF250 by western blot. Samples: Whole cell lysate (50 µg) from F9, NIH 3T3, A20, CH27, and ND7/23 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806458) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human ARID1A/BAF250 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, RKO, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806458) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.