General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit

Product Overview

• Article Name: General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit
• Biozol Catalog Number: BYT-ORB1806532
• Supplier Catalog Number: orb1806532
• Alternative Catalog Number: BYT-ORB1806532-48,BYT-ORB1806532-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Species Reactivity: All
• Alternative Names: 25-HVD3, 25HVD3, Calcifediol, Calcidiol, H-VD3, 25-Hydroxycholecalciferol, 25-Hydroxy Vitamin D3, 25(OH)D

Product Description

General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit

Product Properties

• Range: 3.13-200ng/mL
• Sensitivity: 0.91 ng/mL
• Samples: serum, plasma and other biological fluids
• Target: HVD3

Application Notes

• Application Notes: Application Notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-Hydroxyvitamin D3(HVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-Hydroxyvitamin D3(HVD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of 25-Hydroxyvitamin D3(HVD3) in the samples is then determined by comparing the OD of the samples to the standard curve

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