General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit

Catalog Number: BYT-ORB1806543
Article Name: General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit
Biozol Catalog Number: BYT-ORB1806543
Supplier Catalog Number: orb1806543
Alternative Catalog Number: BYT-ORB1806543-48,BYT-ORB1806543-96
Manufacturer: Biorbyt
Category: Kits/Assays
Species Reactivity: All
Alternative Names: 8-EpiPGF2-alpha, 8epi-PGF2a
General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit
Range: 31.25-2000pg/mL
Sensitivity: 9.55 pg/mL
Samples: serum, plasma and other biological fluids
Target: 8-epi-PGF2a
Application Notes: Application Notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a) in the samples is then determined by comparing the OD of the samples to the standard curve
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