General GonadotropinReleasing Hormone (GnRH) ELISA Kit
Biozol Catalog Number:
BYT-ORB1806567
Supplier Catalog Number:
orb1806567
Alternative Catalog Number:
BYT-ORB1806567-48,BYT-ORB1806567-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Species Reactivity:
All
Alternative Names:
GNRH1, GRH, HH12, LHRH, LNRH, Luliberin
General GonadotropinReleasing Hormone (GnRH) ELISA Kit
Range:
15.63-1000pg/mL
Sensitivity:
9.38 pg/mL
Samples:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Target:
GnRH
Application Notes:
Application Notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with GonadotropinReleasing Hormone(GnRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GonadotropinReleasing Hormone(GnRH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of GonadotropinReleasing Hormone(GnRH) in the samples is then determined by comparing the OD of the samples to the standard curve
orb1806567
orb1806567
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