CEACAM5 Mouse Monoclonal Antibody, Clone: [3C12], Unconjugated

Product Overview

• Article Name: CEACAM5 Mouse Monoclonal Antibody, Clone: [3C12], Unconjugated
• Biozol Catalog Number: BYT-ORB1816926
• Supplier Catalog Number: orb1816926
• Alternative Catalog Number: BYT-ORB1816926-100,BYT-ORB1816926-200,BYT-ORB1816926-50
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Recombinant human CEACAM5 protein
• Conjugation: Unconjugated
• Alternative Names: Carcinoembryonic antigen, Carcinoembryonic antigen related cell adhesion molecule 5, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e, CD66e antigen, CEACAM5, CEAM5_HUMAN, DKFZp781M2392, Meconium antigen 100.

Product Description

CEACAM5 Mouse Monoclonal Antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/ml
• Clone Designation: [3C12]
• Molecular Weight: 180-200 kDa
• UniProt: P06731
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: CEACAM5

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (human appendix), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human esophagus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CEACAM5) Monoclonal Antibody, Unconjugated (orb1816926) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Human LOVO cell lysates, Lane 2: Recombinant human CEACAM5 protein, C-His, Primary: Anti-CEACAM5 (orb1816926) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 77 kDa, Observed band size: 200, 140 kDa.