N-Myc Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: N-Myc Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB1816965
• Supplier Catalog Number: orb1816965
• Alternative Catalog Number: BYT-ORB1816965-100,BYT-ORB1816965-200,BYT-ORB1816965-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human n-Myc (1-100/464aa)
• Conjugation: Unconjugated
• Alternative Names: bHLHe37, Class E basic helix-loop-helix protein 37, MODED, MYCN, MYCN_HUMAN, N myc, N myc proto oncogene protein, N-myc proto-oncogene protein, Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene, Neuroblastoma MYC oncogene, NMYC, NMYC proto oncogene protein, ODED, Oncogene NMYC, pp65/67, v myc avian myelocytomatosis viral related oncogene neuroblastoma derived, v myc myelocytomatosis viral related oncogene neuroblastoma derived.

Product Description

N-Myc Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 49 kDa
• UniProt: P04198
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: MYCN

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Myc) Polyclonal Antibody, Unconjugated (orb1816965) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Myc) Polyclonal Antibody, Unconjugated (orb1816965) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Myc) Polyclonal Antibody, Unconjugated (orb1816965) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Myc) Polyclonal Antibody, Unconjugated (orb1816965) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Myc) Polyclonal Antibody, Unconjugated (orb1816965) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Myc) Polyclonal Antibody, Unconjugated (orb1816965) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Myc) Polyclonal Antibody, Unconjugated (orb1816965) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Cerebellum tissue lysates, Lane 5: Human SH-SY5Y cell lysates, Lane 6: Human U266 cell lysates, Lane 7: Human HeLa cell lysates, Primary: Anti-n-Myc (orb1816965) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kDa, Observed band size: 50 kDa.