GRP78 Antibody (Biotin), Clone: [3C5-1A4], Mouse, Monoclonal

Product Overview

• Article Name: GRP78 Antibody (Biotin), Clone: [3C5-1A4], Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB181919
• Supplier Catalog Number: orb181919
• Alternative Catalog Number: BYT-ORB181919-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: ELISA, ICC, IF, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Full-length recombinant rat GRP78
• Conjugation: Biotin
• Alternative Names: GRP78, GRP-78, Grp78, GRP 78, HSPA5, HSPA 5, Heat shock 70 kDa protein 5, Heat Shock 70kDa Protein 5, BiP, BIP, Binding immunoglobulin protein, Immunoglobulin heavy chain-binding protein, Immunoglobulin Heavy Chain Binding Protein, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Endoplasmic reticulum lumenal, Ca2+ binding protein grp78, Glucose Regulated Protein 78kDa, 78 kDa glucose-regulated protein, 78 kDa glucose regulated protein, HSCe70, MIF2, mBiP, Sez7, AL022860, AU019543, D2Wsu141e, D2Wsu17e, FLJ26106, GRP78_HUMAN

Product Description

Mouse monoclonal antibody against GRP78, conjugated to Biotin.

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [3C5-1A4]
• Molecular Weight: 78kDa
• NCBI: 037215
• UniProt: P06761
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target: GRP78

Application Notes

• Application Dilute: WB (1:1000), ICC/IF (1:100)
• Application Notes: Application Notes: 1 µg/ml of SMC-210 was sufficient for detection of GRP78 in 20 µg of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

Images

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Western Blot analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.

ELISA analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody.