GABA A Receptor Antibody (Biotin), IgG1, Clone: [N87/25 Formerly sold as S87-25], Mouse, Monoclonal

Product Overview

• Article Name: GABA A Receptor Antibody (Biotin), IgG1, Clone: [N87/25 Formerly sold as S87-25], Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB182044
• Supplier Catalog Number: orb182044
• Alternative Catalog Number: BYT-ORB182044-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: AM, ICC, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Fusion protein amino acids 370-433 of mouse GABA-A-R-Beta 3
• Conjugation: Biotin
• Alternative Names: ECA5, GABA alpha receptor beta-2 subunit, GABA(A) receptor subunit beta-3, GABAA receptor beta 3 subunit, GABAA receptor subunit beta 3, GABR B3, GABRB3, Gamma aminobutyric acid (GABA) A receptor beta 3, Gamma aminobutyric acid receptor subunit beta 3, Gamma-aminobutyric acid receptor subunit beta-3, GBRB3_HUMAN, MGC9051

Product Description

Mouse monoclonal antibody against GABA-A Receptor Beta3, conjugated to Biotin.

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [N87/25 Formerly sold as S87-25]
• Molecular Weight: 55kDa
• Isotype: IgG1
• NCBI: 032097
• UniProt: P63080
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target: GABA A Receptor

Application Notes

• Application Dilute: WB (1:1000), IHC (1:1000), ICC/IF (1:100)
• Application Notes: Application Notes: 1 µg/ml of SMC-339 was sufficient for detection of Beta 3 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody

Images

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody, Clone N87/25. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain, Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Beta 3 Antibody (D) Composite.

Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N87/25. Tissue: Brain. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.

Western Blot analysis of Rat brain membrane lysate showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N87/25. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.