Tau (K18) P301L Mutant Pre-formed Fibrils

Product Overview

• Article Name: Tau (K18) P301L Mutant Pre-formed Fibrils
• Biozol Catalog Number: BYT-ORB1822356
• Supplier Catalog Number: orb1822356
• Alternative Catalog Number: BYT-ORB1822356-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: In vitro, In vivo, SDS-PAGE, WB
• Species Reactivity: Human
• Alternative Names: Tau PFFs, Tau PFF, Tau protein Pre-formed Fibrils, Tau aggregates, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 4, tubulin-associated unit

Product Description

Human Recombinant Tau (K18) P301L Mutant PFFs

Product Properties

• Concentration: 2 mg/ml
• Molecular Weight: ~15.1 kDa
• UniProt: P10636
• Buffer: 10 mM HEPES, 100 mM NaCl pH 7.4
• Source: Human
• Purity: >95%
• Sequence: SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE
• Target: Tau (K18) P301L PFFs

Application Notes

• Application Notes: Biological Origin: Human. Biological Activity: Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers. Application Notes: Certified >95% pure using SDS-PAGE analysis. Made with low endotoxin monomer

Images

TEM of recombinant Tau (K18), P301L mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV=80kV.

TEM of recombinant Tau (K18), P301L mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV=80kV.

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau Pre-formed Fibrils. Tau Pre-formed Fibrils seed the formation of new tau fibrils when combined with tau monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.

SDS-PAGE of ~15 kDa Active Human Tau Protein K18 P301L Pre-formed Fibrils. Lane 1: MW Ladder. Lane 2: Tau Protein Pre-formed Fibrils.