Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils

Product Overview

• Article Name: Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils
• Biozol Catalog Number: BYT-ORB1822357
• Supplier Catalog Number: orb1822357
• Alternative Catalog Number: BYT-ORB1822357-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: In vitro, In vivo, SDS-PAGE, WB
• Species Reactivity: Human
• Alternative Names: Tau PFFs, Tau PFF, Tau protein Pre-formed Fibrils, Tau aggregates, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Tau-441, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 2, tubulin-associated unit

Product Description

Human Recombinant Tau-441 (2N4R) P301S Mutant Protein Pre-formed Fibrils

Product Properties

• Concentration: 2 mg/ml
• Molecular Weight: ~45.8 kDa
• UniProt: P10636
• Buffer: 10 mM HEPES, 100 mM NaCl pH 7.4
• Purity: >95%
• Sequence: MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGKQAA AQPHTEIPEG TTAEEAGIGD TPSLEDEAAG HVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPP GQKGQANATR IPAKTPPAPK TPPSSGEPPK SGDRSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TP
• Target: Tau-441 (2N4R) P301S Mutant

Application Notes

• Application Notes: Biological Activity: Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers. Application Notes: Certified >95% pure using SDS-PAGE analysis. Low endotoxin <5 EU/mL 2mg/mL

Images

TEM of recombinant Tau441 (2N4R), P301S mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV = 80kV. Fibrils were sonicated and stained with uranyl acetate.

TEM of recombinant Tau441 (2N4R), P301S mutant Pre-formed Fibrils (PFFs). Fibrils were sonicated and stained with uranyl acetate.

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) when tau Pre-formed Fibrils are combined with tau monomers. The Pre-formed Fibrils seed the formation of new fibrils from a pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.

SDS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Pre-formed Fibrils. Lane 1: MW Ladder. Lane 2: Tau Protein Pre-formed Fibrils