Tau (K18) P301L Mutant Monomers

Product Overview

• Article Name: Tau (K18) P301L Mutant Monomers
• Biozol Catalog Number: BYT-ORB1822358
• Supplier Catalog Number: orb1822358
• Alternative Catalog Number: BYT-ORB1822358-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: In vitro, In vivo, SDS-PAGE, WB
• Species Reactivity: Human
• Alternative Names: Tau monomer, Tau protein monomer, Tau protein, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Tau-441, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 4, tubulin-associated unit

Product Description

Human Recombinant Tau (K18) P301L Mutant Monomers

Product Properties

• Concentration: 2 mg/ml
• Molecular Weight: ~15.1 kDa
• UniProt: P10636
• Buffer: 10 mM HEPES, 100 mM NaCl pH 7.4
• Source: Human
• Purity: >95%
• Sequence: SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE
• Target: Tau (K18) P301L Monomers

Application Notes

• Application Notes: Biological Origin: Human. Biological Activity: Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when tau PFFs are combined with tau monomers. Application Notes: Certified >95% pure using SDS-PAGE analysis. Low endotoxin <5 EU/mL 2mg/mL

Images

SDS-PAGE of ~16 kDa Human Tau Protein K18 P301L Monomer. Lane 1: MW Ladder. Lane 2: Tau Protein Monomer

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) in tau K18 P301L monomers over time. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.