Tau-441 (2N4R) P301S Mutant Monomers

Product Overview

• Article Name: Tau-441 (2N4R) P301S Mutant Monomers
• Biozol Catalog Number: BYT-ORB1822359
• Supplier Catalog Number: orb1822359
• Alternative Catalog Number: BYT-ORB1822359-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: In vitro, In vivo, SDS-PAGE, WB
• Species Reactivity: Human
• Alternative Names: Tau monomer, Tau protein monomer, Tau protein, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Tau-441, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 2, tubulin-associated unit

Product Description

Human Recombinant Tau-441 (2N4R) P301S Mutant Monomers

Product Properties

• Concentration: 2 mg/ml
• Molecular Weight: ~45.8 kDa
• UniProt: P10636
• Buffer: 10 mM HEPES, 100 mM NaCl pH 7.4
• Source: Human
• Purity: >95%
• Sequence: MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGKQAA AQPHTEIPEG TTAEEAGIGD TPSLEDEAAG HVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPP GQKGQANATR IPAKTPPAPK TPPSSGEPPK SGDRSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TP
• Target: Tau-441 (2N4R) P301S Monomers

Application Notes

• Application Notes: Biological Origin: Human. Biological Activity: Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when tau PFFs are combined with tau monomers. Application Notes: Certified >95% pure using SDS-PAGE analysis. Low endotoxin <5 EU/mL 2mg/mL

Images

SDS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Monomer. Lane 1: MW ladder. Lane 2: Tau Protein Monomer

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau monomers. A greater increase in fluorescence is seen when 50 uM monomer is combined with 10 nM PFFs, as the fibrils seed the formation of new fibrils from the pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.